Applied Biosystems THERMALCYCLER N8050200 – Dual 96-Well PCR Thermal Cycler | GeneAmp / ProFlex Platform
Engineering Background
The Applied Biosystems Dual 96-Well Thermal Cycler (base unit N8050200) is a high-throughput PCR platform engineered for molecular biology, genomics, and clinical research laboratories. Combining two independent 96-well blocks in a single chassis, this system enables simultaneous execution of two distinct PCR protocols—maximizing instrument utilization without compromising thermal precision.
Product Positioning
Designed for procurement engineers and lab managers sourcing verified surplus or refurbished capital equipment, this unit is offered through Konmask.com with full part-number traceability across all major sub-assemblies: 0100-09086 (block assembly), 0040-09271 (lid/heated cover), 0010-09341 (control PCB), and 0100-94029 (power supply module). Ideal for high-volume genotyping, endpoint PCR, and colony screening workflows.
Complete Technical Specifications
| Parameter | Specification |
|---|---|
| Model / Base Part No. | THERMALCYCLER N8050200 |
| Block Configuration | Dual 96-Well (2 × 96 × 0.2 mL) |
| Sample Capacity | 192 wells total (2 independent blocks) |
| Temperature Range | 4 °C – 99 °C |
| Heating Rate | Up to 3.3 °C/s (block-dependent) |
| Cooling Rate | Up to 2.0 °C/s |
| Temperature Uniformity | ±0.25 °C across block at 72 °C |
| Heated Lid Temperature | 50 °C – 110 °C (adjustable) |
| Compatible Consumables | 0.2 mL tubes, 96-well plates, strip tubes |
| Sub-Assembly Part Nos. | 0100-09086 / 0040-09271 / 0010-09341 / 0100-94029 |
| Power Input | 100–240 V AC, 50/60 Hz |
| Weight | ~4.1 kg |
| Platform | Applied Biosystems GeneAmp / ProFlex Series |
Proven Application Environments
- High-throughput genotyping and SNP analysis
- Endpoint PCR and colony screening
- cDNA synthesis and RT-PCR workflows
- Forensic DNA amplification
- Pharmaceutical QC and bioanalytical labs
- University and government research institutions
- CRO / CDMO multi-instrument PCR suites
Engineering Advantages
- Dual independent blocks – run two different protocols simultaneously with zero cross-contamination risk
- Precise thermal uniformity – ±0.25 °C ensures reproducible amplification across all 96 wells per block
- Adjustable heated lid – eliminates condensation without manual intervention
- Full sub-assembly traceability – all four part numbers documented for maintenance and compliance records
- Universal voltage input – 100–240 V AC supports global lab deployment
- Compact dual-block footprint – maximizes bench space efficiency vs. two standalone units
System Architecture & Signal Flow
The N8050200 chassis integrates two thermally isolated Peltier-based 96-well blocks controlled by an independent microprocessor per block (control PCB: 0010-09341). The heated lid assembly (0040-09271) applies uniform downward pressure and thermal compensation to prevent evaporation. The power supply module (0100-94029) delivers regulated DC rails to both Peltier arrays and the control electronics. Block assembly 0100-09086 houses the silver-block heat sink and temperature sensor array, enabling closed-loop PID thermal control with sub-second response.
Sourcing & Quality Verification
- Unit sourced from decommissioned research-grade laboratory environments
- All four sub-assembly part numbers verified against Applied Biosystems OEM documentation
- Visual and functional inspection completed prior to listing
- Weight verified at 4,100 g — consistent with factory specification
- Konmask.com maintains full chain-of-custody records available upon request
Procurement Process
- Submit inquiry via support@konmask.com or call +0086 19859288691
- Receive proforma invoice with full part-number breakdown within 1 business day
- Confirm payment — T/T, PayPal, or platform checkout accepted
- Unit ships within 3–5 business days with tracking; export documentation available for international orders
- Post-delivery technical support provided by Konmask engineering team
Technical FAQ
Q: Are all four part numbers (0100-09086, 0040-09271, 0010-09341, 0100-94029) present in this unit?
A: Yes. All sub-assemblies are accounted for and documented in the listing.
Q: Can this unit run two different annealing temperatures simultaneously?
A: Yes. Each block operates independently with its own thermal program and temperature setpoints.
Q: Is a calibration certificate available?
A: Functional verification data is available. Third-party NIST-traceable calibration can be arranged at additional cost.
Q: What is the lead time for international shipping?
A: Typically 7–14 business days depending on destination. Export documentation (commercial invoice, packing list) is provided as standard.
Q: Is this compatible with Applied Biosystems MicroAmp plates?
A: Yes. Standard 0.2 mL 96-well plates and MicroAmp-format consumables are fully compatible.
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Contact: support@konmask.com | +0086 19859288691
